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A method to characterize the magnetic moment of a magnetic force microscopy (MFM) tip is presented. The response of the MFM while imaging a superparamagnetic film is compared to a nonmagnetic reference. A simple model is used to compare the experimental results with a calculation of the response of the MFM tip due to the interactions with the magnetization of a superparamagnetic film. © 2004 American Institute of Physics.