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A digital holographic method for three-dimensional reconstruction of tomography images is demonstrated theoretically and experimentally. In this proposed method, a numerical hologram is first computed by calculating the total diffraction field of all transect images of a detected organ. Then, the numerical hologram is transferred to the usual recording medium to generate a physical hologram. Last, all the transect images are reconstructed in their original position by illuminating the physical hologram with a laser, thereby forming a three-dimensional transparent image of the organ detected. Due to its true third dimension, the reconstructed image using this method is much more vivid and accurate than that of other methods. Potentially, it may have great prospects for application in medical engineering. © 2004 American Institute of Physics.