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Optically driven micromanipulators with submicron probe tips are proposed and developed by using two-photon microstereolithography. The micromanipulators are worked by maneuvering their movable component with a focused laser beam, and an actual pair of microtweezers was opened and shut precisely. We also propose an effective method of controlling movable micromachines with great freedom of movement. In this method, a dot is attached to a movable component for trapping and driving it by a single laser beam. A microneedle was induced to perform several types of motion such as rotation and translation. The optically driven micromanipulators are useful for bionanotechnology applications that require work to be done in aqueous solutions. © 2003 American Institute of Physics.