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Electrical control of loaded biomimetic femtoliter vesicles in microfluidic system

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2 Author(s)
Tresset, G. ; Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos #04-01, Singapore 138669, Singapore ; Iliescu, C.

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This letter describes how femtoliter lipid vesicles encapsulating a fluorescent marker can be aligned, porated, and fused by electric field in microfluidic system. Vesicles are prepared with a soluble dye and filtrated through a size-exclusion column. After introduction into a microdevice comprising heavily doped silicon electrodes sealed between glass substrates, the vesicles are arranged in “pearl chain” clusters under the application of an external ac voltage. Release of the encapsulated dye upon the application of high electric field pulses reveals the formation of submicrometer pores in the membrane, and the fusion of distinct vesicles can also be achieved.

Published in:

Applied Physics Letters  (Volume:90 ,  Issue: 17 )

Date of Publication:

Apr 2007

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