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The focusing capabilities of a two-dimensional fluid-filled lens microfabricated in an optical polymer are demonstrated. The illumination path is visualized by localized scattering centers. Functionality for flow cytometry applications is demonstrated by localization of the excitation of large-angle scatter. Integrated in-plane optical systems offer simple, compact, and inexpensive alternatives to external optics, as well as the potential for increased detection efficiency and low-power operation.