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Construction of three-dimensional biomolecule structures employing femtosecond lasers

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8 Author(s)
Drakakis, Theodore S. ; Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology Hellas (FORTH), P.O. Box 527, Heraklion, Crete 711 10, Greece and Department of Physics, University of Crete, Crete 71409, Greece ; Papadakis, G. ; Sambani, Kyriaki ; Filippidis, George
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The authors demonstrate here a method for three-dimensional patterning of proteins and other biological molecules. The method employs femtosecond-laser-induced three-photon polymerization, a technique which enables the construction of arbitrary two- and three-dimensional structures of submicron resolution. Biotin is subsequently attached to the three-dimensional (3D) structures via UV-activated cross-linking. The integrity of the photolytically immobilized biotin is confirmed by detecting the binding of fluorescently labeled avidin via fluorescence microscopy and via a surface acoustic sensor technique. In all, the technique opens the way for the fabrication of structures with a wide range of biomaterials as well as studying their dynamics within complex 3D structures.

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Applied Physics Letters  (Volume:89 ,  Issue: 14 )