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Localization and cross section reconstruction of fluorescent targets in ex vivo breast tissue using independent component analysis

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4 Author(s)
Alrubaiee, M. ; Institute for Ultrafast Spectroscopy and Lasers, Physics Department, The City College of New York of the City University of New York, 138th Street, Convent Avenue, New York, New York 10031 ; Xu, M. ; Gayen, S.K. ; Alfano, R.R.

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An information theory based approach is introduced for the detection and three-dimensional localization of fluorescent targets embedded in a turbid medium. The approach uses multisource illumination of the medium, multidetector transillumination acquisition of fluorescence signal, independent component analysis for target detection and localization, and a back-projection algorithm for reconstruction of target cross section. The efficacy of the approach is demonstrated by locating and estimating the cross section of a fluorescent target embedded in a 100×100×26 mm3 ex vivo human breast tissue specimen and in a tissue-simulating turbid medium of thickness 50 times the transport mean free path.

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Applied Physics Letters  (Volume:89 ,  Issue: 13 )