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Gold nanoparticle arrays are fabricated on a planar gold surface obtained from UV-photopatterned self-assembled monolayer (SAM) films. The arrays are composed of microdots in which spherical gold nanoparticles are immobilized above a gold surface with a gap distance of a few nanometers supported by SAM films used as a spacer. Two kinds of preparation methods were examined: (1) to pattern a spacer SAM film that makes cross-linkage between the gold surface and the gold nanoparticles, and (2) to form ordered microholes in a SAM film, followed by deposition of the spacer SAM film that makes the cross-linkage in the microholes. Both methods provide us with the ordered microdots of gold nanoparticles on a gold surface. The formation of the microdot arrays were probed by optical second-harmonic microscopy.