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Three-dimensional confocal thermal imaging using anti-Stokes luminescence

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4 Author(s)
Kachynski, A.V. ; The Institute for Lasers, Photonics, and Biophotonics, Department of Chemistry, SUNY at Buffalo, Buffalo, New York 14260-3000 ; Kuzmin, A.N. ; Pudavar, H.E. ; Prasad, P.N.

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High-resolution thermal images of dye-stained live cells are obtained using confocal anti-Stokes luminescence on a confocal laser scanning microscope. Stokes and anti-Stokes fluorescence signal ratio imaging, using dual laser excitation with a fast alternate line scan, is used to monitor dynamical temperature changes at submicrometer resolution. Temperature mapping of a dye-doped polymer film with local heating is shown as an example. Three-dimensional mapping of local temperature changes in living cells induced by an external laser as the heating source, is obtained with temperature resolution better than 0.5 K.

Published in:

Applied Physics Letters  (Volume:87 ,  Issue: 2 )