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We have presented an early detection system, ZASMIN (Zero-day Attack Signature Management Infrastructure), for novel network attack protection. This system provides early detection function and validation of attack at the moment the attacks start to spread on the network. In order to detect unknown network attack, the ZASMIN system has adopted various of new technologies, which are composed of suspicious traffic monitoring, attack validation, polymorphic worm recognition, signature generation. Some of these functionalities are implemented with hardware-based accelerator to be able to deal with giga-bit speed traffic, therefore, it can be applicable to Internet backbone or the bottle-neck point of high-speed enterprise network without any loss of traffic. In order to check the feasibility of ZASMIN, we have installed it on real honeynet environment, then we have analyzed the result about detection of unknown attack.