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This paper presents a novel approach to simulate a Knowledge Based System for diagnosis of Breast Cancer using Soft Computing tools like artificial neural networks (ANNs) and Neuro Fuzzy Systems. The feed-forward neural network has been trained using three ANN algorithms, the back propagation algorithm (BPA), the radial basis function (RBF) Networks and the learning vector quantization (LVQ) Networks; and also by adaptive neuro fuzzy inference system (ANFIS). The simulator has been developed using MATLAB and performance is compared by considering the metrics like accuracy of diagnosis, training time, number of neurons, number of epochs etc. The simulation results show that this Knowledge Based Approach can be effectively used for early detection of Breast Cancer to help oncologists to enhance the survival rates significantly.