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Flow-Lysometry and its Biomedical Application: Cytosolic Analysis of Single Cells in Large Populations

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4 Author(s)
W. C. Lee ; Red Cell Membrane Laboratory, Children's Hospital Oakland Research Institute, California, USA; Berkeley Sensor & Actuator Center, UC Berkeley, California, USA ; F. A. Kuypers ; Y. -H. Cho ; A. P. Pisano

We present a novel single-cell analyzer, flow-lysometry, to measure cytosolic components in large cell populations. The present flow-lysometry performs and synchronizes three functions (cell-detection, cell-lysis, and component-sensing) in a continuous microfluidic channel, thus achieving high-throughput measurements (20 cells/min) of wide target components. In the experimental study, we measure the cytosolic component (Ca++ ion) from each single RBC and verify population analysis of single cells in mixed cell populations. Thus, this work shows that the present flow-lysometry is useful to characterize complex cell populations, which is required for various biomedical studies.

Published in:

Micro Electro Mechanical Systems, 2009. MEMS 2009. IEEE 22nd International Conference on

Date of Conference:

25-29 Jan. 2009