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Tissue-motions can represent the strength of artery pulsation of a newborn baby for pediatrics diagnosis. In this paper, we propose a novel method to estimate the tissue-motion quantitatively by combining local and global motion estimation methods, namely combined local-global (CLG) optical flow technique in cranial ultrasonogram of newborn babies. Tissue motions are estimated in different coronal sections successfully. Further, we analyze the time variant tissue-motion by using discrete Fourier transform. We also observed the pulsation in the time variant motion images and strong pulsation is observed in the harmonic frequency of tissue-motion that has a relation to the heartbeat frequency of a newborn baby.