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Rapid Specific and Label-Free Detection of Porcine Rotavirus Using Photonic Crystal Biosensors

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6 Author(s)
Pineda, M.F. ; Dept. of Bioeng., Univ. of Illinois at Urbana-Champaign, Urbana, IL ; Leo Li-Ying Chan ; Kuhlenschmidt, T. ; Choi, C.J.
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A rapid, label-free assay using photonic crystal biosensors for the direct detection of intact viable rotaviruses is demonstrated. This paper describes the design and operation of the biosensor, surface functionalization with anti-rotavirus antibody, characterization of the detection sensitivity, and demonstration of specificity with respect to non-target virus particles. A 30-min assay of a partially processed water sample yielded a detection sensitivity of 36 virus focus forming units without the use of any external reagents. This sensitivity is comparable to commercial enzyme-linked immunosorbent assays. These results suggest photonic crystal biosensors may be useful for real-time monitoring of virus contamination of environmental water resources.

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Sensors Journal, IEEE  (Volume:9 ,  Issue: 4 )