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This paper describes the development of a screen-printed immunosensor. Special attention is paid to the optimization that is achievable by modifying certain technological parameters, such as distance, surface, width, or temperature. Hints are also given on how to produce a simple, cheap, and reliable measurement system that is suitable to analyze the results provided by the immunosensors developed. The results presented throughout this paper will be used to develop a screen-printed immunosensor whose performance will be compared with that of the one obtained from a commercial sensor.