Cart (Loading....) | Create Account
Close category search window
 

Immunonanogold-Catalytic Cu _{2} O-Enhanced Assay for Trace Penicillin G With Resonance Scattering Spectrometry

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

4 Author(s)
Zhiliang Jiang ; Guangxi Key Lab. of Environ. Eng., Protection & Assessment, Guangxi Normal Univ., Guilin ; Aihui Liang ; Yan Li ; Xiaoling Wei

A novel immunonanogold-catalytic Cu2O-enhanced resonance scattering (RS) spectral assay is reported, based on nanogold modified rabbit anti-penicillin G (RAPG) antibody, its catalytic enhanced effect on the slow particle reaction between Cu(II) and glucose, and the RS effect of (Au)nucleus(Cu2O)shell complex particle at 608 nm. As a model, we used nanogold in size of 9 nm to label RAPG to obtain an immunonanogold probe (AuRAPG) for penicillin G (PG). The immunoreactions between the probe and PG took place in phosphate-citric acid buffer solutions. After centrifugation, the excess AuRAPG in the supernatant was used to catalyze the particle reaction to amplify the RS signal. With the addition of PG, the concentration of AuRAPG in the supernatant reduced linearly, which leaded the I608 nm to decrease accordingly. The decreased RS intensity was proportional to the PG concentration in the range of 0.09-21.6 ngldrmL-1, with a detection limit (DL) of 0.01 ngldrmL-1 PG. The assay was used to determine trace PG in raw milk sample, with the recovery between 100.6% and 109.2%.

Published in:

NanoBioscience, IEEE Transactions on  (Volume:7 ,  Issue: 4 )

Date of Publication:

Dec. 2008

Need Help?


IEEE Advancing Technology for Humanity About IEEE Xplore | Contact | Help | Terms of Use | Nondiscrimination Policy | Site Map | Privacy & Opting Out of Cookies

A not-for-profit organization, IEEE is the world's largest professional association for the advancement of technology.
© Copyright 2014 IEEE - All rights reserved. Use of this web site signifies your agreement to the terms and conditions.