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This paper presents a microfluidic device for magnetophoretic separation red blood cells from blood under continuous flow. The separation method consist of continuous flow of a blood sample (diluted in PBS) through a microfluidic channel which presents on the bottom ldquodotsrdquo of ferromagnetic layer. By applying a magnetic field perpendicular on the flowing direction, the ferromagnetic ldquodotsrdquo generate a gradient of magnetic field which amplifies the magnetic force. As a result, the red blood cells are captured on the bottom of the microfluidic channel while the rest of the blood is collected at the outlet. Experimental results show that an average of 95 % of red blood cells is trapped in the device.