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Array arrangement of living cells on self-assembled-monolayer pattern chip with femtosecond laser inducing mechanical force "micro tsunami"

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7 Author(s)
Kira, A. ; R&D Div., ULVAC, Inc, Chigasaki ; Okano, K. ; Hosokawa, Yoichiroh ; Fuwa, K.
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We have developed a novel method for arranging living cells on a glass chip with micrometer-scale cell-adhering areas by applying femtosecod laser. To form the cell-adhesion area, the chip surface was first covalently modified with perfluoroalkyl self-assembled-monolayer (Rf-SAM) and then etched by oxygen-plasma. The Rf-surface and the etched surface were characterized using solvent contact-angle analysis. It was confirmed that the Rf-surface has low surface-free-energy as contact angle was 118.2deg and 69.0deg with water and hexadecane, respectively. Thus the Rf-surface has repellent characteristics for polar and nonpolar materials. Protein adsorption on the fabricated Rf-micropattern chip was evaluated using R-phycoerythrin. There was little signal derived from R-phycoerythrin on the Rf-surface indicating that Rf-SAM inhibits the protein adsorption onto the surface. When mammalian PC12 cells were cultured on the Rf-micropattern chip, the cells adhered and grew up only on the etched glass surface but not on Rf-surface. Cultured cells were detached one by one from a culture substrate and transported onto a specific cell-adhering area on the Rf-micropattern chip by using femtosecond-laser-induced mechanical force that we named "micro tsunami".

Published in:

Micro-NanoMechatronics and Human Science, 2008. MHS 2008. International Symposium on

Date of Conference:

6-9 Nov. 2008