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Artery models and surgical simulators are required for safety surgery. We have proposed a novel method to produce biological models using polyurethane elastomer, which has high photoelastic coefficient. And a method is to analyze stress on the model by circular polarized light method. the quantitative stress is analyzed from rainbow-colored photoelastic pattern. But the measurement area is restricted for the fringe area of the artery model. In this paper, we propose stress measurement on biological model by reflection method, which is a stress measurement method using photoelastic effect. With this method, stress is measured in area wider than that of circular polarized light method. For the reflection method, the Reflection layer was coated between first layer of polyurethane elastomer and second layer of it. From SEM image of cross section of this artery model image, we confirmed uniform thickness of acrylic resin and adhesion between polyurethane layer and acrylic layer. To use this reflection model, We could quantify the error of photo elastic stress analysis. the measurement accuracy of stress state of model reflection area was found to be 9.1% about stress magnitude and plusmn 7[deg] about stress directions. And an application on artery model is verified experimentally. Finally, Stress on the vascular wall caused by the contact with a catheter was successfully measured. Consequently, it should be valuable for various applications not only surgical simulations but also hemodynamic studies and pathological studies.