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Three dimensional spheroids formation platform using micro-rotation flow

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5 Author(s)
Ota, H. ; Sch. of Integrated Design Eng., Keio Univ., Yokohama ; Deguchi, K. ; Kita, S. ; Yamamoto, R.
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Hepatocytes are responsible for metabolic and detoxification processes in the liver. During the tissue formation from isolated cells, they increase the cell density and acquire their liver-specific functions, new protein expression and cell signaling. Hence, tissue-based in vitro studies are strongly demanded. We develop a MEMS platform which forms 3D spheroids by microfluodic handing of cells. The microfludic platform is composed of PDMS chamber fabricated by microlithography and a circulation system. We simulated the flow in the chamber by FLUENT and found that micro-rotation flow was generated when the chamber height was four times greater than the channel height. The rotation flow was experimentally observed using fluorescent microbeads with the newly designed micro-chamber. The proposed MEMS-based 3D-spheroids formation platform is readily to create a technological innovation in biology as a bridge between cell researches and tissue researches.

Published in:

Micro-NanoMechatronics and Human Science, 2008. MHS 2008. International Symposium on

Date of Conference:

6-9 Nov. 2008