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Scanning confocal near-infra-red microscopy: a new microscopy technique for three-dimensional histopathology

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8 Author(s)
Murthy, M.S.N. ; Dept. of Clinical Radiol., Bristol Univ., UK ; Jones, M.G. ; Kulka, J. ; Davies, J.D.
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To image microstructures within tissue in detail using conventional light microscopy it is necessary to process the tissue and obtain thin (≈5 μm) physical sections by using destructive physical sectioning techniques. This article describes a microscopy technique, using near-infra-red light (700-1500 nm) as the source, which is able to image thin sections of thick-tissue specimens nondestructively. Images of contiguous two-dimensional thin sections obtained serially can be combined to generate a three-dimensional image of the structures being imaged

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Engineering Science and Education Journal  (Volume:4 ,  Issue: 5 )