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A direct-current cold atmospheric-pressure air plasma microjet (PMJ) based on the microhollow cathode discharge design is used to inactivate six types of bacteria within a small well-defined area on a large Petri dish. We show that the PMJ is very effective in inactivating bacteria in their vegetative state as well as in the spore state within the area of plasma exposure. We also observed that bacteria in their vegetative state were inactivated efficiently outside the area of direct plasma exposure. Different bacteria responded differently to an increase in the plasma exposure (dose). Lastly, we observed two types of colony forming unit (CFU) distributions after plasma treatment; one distribution is diffusionlike with a gradual increase of the surviving CFU as one moves radially away from the area of direct plasma exposure, and the other distribution shows an essentially uniform reduction in surviving CFU across the entire Petri dish.