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DoS attack is a critical threat to Grid Web service. Recently too many technologies of the detection and prevention have developed, but it is difficult that the IDS distinguishes normal traffic from the DoS attack. Therefore, when the DoS attack is detected by IDS, the firewall just discards all over-bounded traffic for a victim of absolutely decreases the threshold of the router. Also, attacker use spoofing IP address. So we introduce DoS vulnerability of SSO and SAML in Web service. To solve DoS vulnerability in Web service, we analyze DoS in SAML and introduce agent to prevent DoS attacks.