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Summary form only given. As number and diversity of data, information and resources increase importance of usage of knowledge for their accessing and reuse grows significantly. One of the most useful approaches is based on semantic description of elementary data and pieces of information as well as on relations between them, therefore resulting in ontological approach. Semantics is starting to be used in wider scope than ever, especially for accessing distributed resources. This field is well represented by the Semantic Web, where WWW content can be expressed also in a format that can be read and used by automated tools, thus permitting people and machines to find, share and integrate information more easily. Semantic description of services and user requests can also be used in (semi-)automatic construction of distributed/Grid applications, often in a form of workflows. In order to effectively manage ontologies on the level higher than the local one, e.g., using Virtual Organization approach some sort of knowledge base must be provided.