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In the present there are many problems in the research of teaching and the application of E-Learning, like theories separated, theoretical basis indefinite, techniques used indiscriminately, interaction lacked, extension of the knowledge and dialectical thoughts insufficient etc. Therefore the ideal effect of knowledge extension could not be reached based on modern instructional theories. The paper combines the course with experience, creatively develops an E-Learning system based on web, and puts the basic theories of E-Learning and technical development into practice synthetically. This paper practices this brand new mode, through the development of E-Learning instructional system, and along with the teaching experience.