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The magnetic anisotropy constants of Fe 3Pt alloy thin films fabricated by e-beam evaporation onto MgO (100), (111), and (110) substrates held at various deposition temperatures were investigated in conjunction with structure. It is observed that the orientation of the Fe3Pt alloy thin films depends on the substrate. The magnetic anisotropy constants of each oriented Fe 3Pt alloy thin films are found to be structure sensitive. The temperature dependence of the magnetic anisotropy constants and magnetization of the Fe3Pt alloy thin films were examined. The dependence for Fe3Pt alloy thin films between the magnetic anisotropy constants and magnetization is consistent with the theoretical prediction of M2 for the two-ion model put forward for the case of the Fe50Pt50 alloy.