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Video transport over multi-hop wireless networks has received significant research interests recently. The majority of the research efforts in this field have been conducted taking the approach of cross-layer optimization. However, video content and user perceived quality have been largely ignored in existing work. In this paper, we integrate video content analysis into video transport over wireless mesh networks (WMN). A content-aware quality-driven cross-layer optimization framework is proposed to achieve the best end-to-end user perceived video quality. In our framework, the extracted video regions of interest (ROI) are discriminatingly coded, transmitted and protected in video encoding, network routing and packet scheduling by different network layers. We aim at the optimization of key parameters of each layer while focusing on their interactions across the holistic network protocol stack. The proposed framework is evaluated by H.264/AVC codec and WMN simulations. Experimental results demonstrate that the proposed framework can effectively provide a good user perceived video quality, especially when the delay requirement is stringent.