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For medical images diagnostically important information often lies in the texture. Fractal dimension may be used as an index of irregularity. In this paper we describe the adaptation of the intensity difference scaling method for assessment of the fractal dimension D in the irregular regions of interest (irregular ROIs). This is of great importance because the investigated regions are often small. It is difficult to fit entire regular region of interest within the examined organ with simultaneous inclusion of the relevant fragment, and at the same time to avoid the influence of boundaries. Fractal analysis of various kinds of medical images: panoramic radiography and nuclear medicine scan showed the validity of assessment of D in irregular ROIs.