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In this paper, we propose a robust narrowband interference excision technique in wireless communications based on modulated lapped biorthogonal transform (MLBT), including the Malvar's MLBT and the MLBT constructed by Matviyenko's optimized window. First, we study the bit error rate (BER) performance of the MLBT-based exciser in direct sequence spread spectrum (DSSS) system theoretically. Then we study the application of MLBT to narrowband interference suppression in orthogonal frequency division multiplexing spread spectrum (OFDM-SS) and multiband (MB)-OFDM-based ultra wideband (UWB) communications by simulations. Simulation results show that the Matviyenko-MLBT-based narrowband excision technique has the improved BER performance and increased robustness with respect to the interference frequency offset relative to the modulated lapped transform (MLT)-based technique in DSSS. It is also superior to the fast Fourier transform (FFT) and the MLT-based interference suppression technique in OFDM-SS and MB-OFDM-based UWB communications.