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In this paper, a novel signal perturbation free transmit scheme is proposed for MIMO channel estimation. A perturbation analysis of the WR-based method is first conducted, showing that the method is subject to a signal perturbation error and therefore, its performance is very poor under the moderate to high signal-to-noise ratios (SNRs). A new transmit structure is then proposed to cancel the signal perturbation error at the receiver in order to improve the performance of the WR-based method in the high SNR case. Computer simulations show that the WR-based method with the proposed signal perturbation free transmit scheme significantly outperforms the original WR-based method as well as the training-based LS method in terms of the MSE of the channel estimate.