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We propose a knowledge-based ubiquitous and persistent sensor network (KUPS) for threat assessment, in which "sensor" is a broad characterization. It refers to diverse data or information from ubiquitous and persistent sensor sources such as organic sensors and human intelligence sensors. Our KUPS for threat assessment consists of two major steps: situation awareness using fuzzy logic systems (FLSs) and threat parameter estimation using radar sensor networks (RSNs). Our FLSs combine the linguistic knowledge from different intelligent sensors, and our proposed maximum-likelihood (ML) estimation algorithm performs target radar cross section (RCS) parameter estimation. We also show that our ML estimator is unbiased and the variance of parameter estimation matches the Cramer-Rao lower bound (CRLB) if the radar pulses follow the Swerling II model. Simulations further validate our theoretical results.