By Topic

Feasibility study of atmospheric pressure plasma treatments of HEPG-2 and SK-HEP-1 cancer cells

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

7 Author(s)
Gweon, B. ; Dept. of Phys., Korea Adv. Inst. of Sci. & Technol., Daejeon ; Kim, Dan Bee ; Moon, S.Y. ; Choe, W.
more authors

Summary form only given. A feasibility study of plasma cancer cell treatment was performed using a pin-type plasma jet. A 50 kHz AC power was used to generate the plasma in the ambient air assisted by the helium flow. The plasma gas temperature was maintained at the room temperature of 25degC to eliminate the thermal damages to the cell. The liver cancer cells of HEPG-2 and SK-HEP-1 were used as the treatment samples. The sample cells were prepared on the fibronectin coated slide glass for the sake of convenience of the microscopic observation process. The input voltage and the treatment time were controlled in the range of (750-1000) V and (30-120) s, respectively. After the plasma treatments, the sample cells were stained with the viability assay (ethidium homodimer and calcein AM) to make discrimination between live and dead cells. Then, the microscopy scan was performed from the plasma center up to about 1 cm. The necrotized cells as well as a void or cell free zone appeared starting at the input voltage of 800 V and the treatment time of 60 s, . The necrotized zone and the void were well matched to the plasma profile. Normal liver cell, THLE-2, was then treated for comparison with the liver cancer cells.

Published in:

Plasma Science, 2008. ICOPS 2008. IEEE 35th International Conference on

Date of Conference:

15-19 June 2008