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Fiber-optic Nanoprobe Measurement of Intracellular pH in Single CA46 Cell Using pH-dependent Dye

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5 Author(s)
Na Fang ; Key Lab. of Optoelectron. Sci. & Technol., Fujian Normal Univ., Fuzhou ; Yishen Qiu ; Zhihao Chen ; Qinmiao Chen
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This paper describes the use of nanosensor and pH-dependent fluorescent dye to monitor intracellular pH in single CA46 cell. The pH-dependent fluorescent dye 5 (6)-Carboxyeosin diacetate (CFDA) was used to measure intracellular pH from the surface fluorescence of the single CA46 Cell. Argon ion laser was focused on single cell with a fiber optic nanoprobe which is parked outside on the single cell, also the emitted light was collected with this fiber optic nanoprobe. To account for system instabilities, we have explored a ratiometric approach to determine the intracellular pH on the single-cell level after staining with CFDA. Calibration of the intracellular fluorescence spectra is obtained by equilibration of internal and external pH with Nigericin in k+ buffers at different external pH. Besides accurate estimated of intracellular pH (7.21 plusmn 0.05). This technique permitted monitor transient changes in intracellular pH caused by addition and removal of NH4 + and allow to study pH-regulatory in Single CA46 cell.

Published in:

2008 International Conference on BioMedical Engineering and Informatics  (Volume:2 )

Date of Conference:

27-30 May 2008