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Image sensor-based polarization analyzing CMOS sensor that will be integrated on the microchamber or microfluidic system is proposed. A CMOS image sensor with 2 x 2 -parallel operation scheme is designed for the polarization analysis. Embedded wire grid polarizer with various grid angles was configured on the photodiodes. The basic function of the polarization analyzing sensor is experimentally demonstrated, too. Polarization profile measurement and polarimetric measurement are successfully demonstrated with a photosensor array designed for the proposed measurement scheme.