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Non-uniform 3D distance transform for anisotropic signal correction in confocal image volumes of skeletal muscle cell nuclei

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2 Author(s)
Edlund, P.K. ; Centre for Image Anal., Uppsala Univ., Uppsala ; Lindblad, J.

Detailed description of the three dimensional organization of nuclei in confocal microscope image volumes of skeletal muscle fibers needs accurate identification of the nuclei centroids. We propose a model based method, applied as a weighted distance transform that take into consideration the diffuse, attenuated, anisotropic, and non-symmetric behavior of the nuclei signals, to provide a transformed image volume where the individual nuclei are better separated, and where the correct nuclei centroids are easily attained.

Published in:

Biomedical Imaging: From Nano to Macro, 2008. ISBI 2008. 5th IEEE International Symposium on

Date of Conference:

14-17 May 2008