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In the study, a selective medium was used to enrich and isolate a naphthalene-degrading bacterium NAP_A from the activated sludge of Zhangdian Municipal Wastewater Treatment Plant in Zibo, China. Cultivation and gas chromatography analysis showed NAP_A degraded naphthalene fast under 30degC~35degC and pH7. The 16S rRNA gene of NAP A was cloned and sequenced (EU142847). Basing on this sequence and its related sequences in the GenBank database, a multialignment was conducted then a phylogenetic tree was constructed with DNAMAN software. The results showed that strain NAP_A was located in the lineage of Ochrobactrum genus, sharing the highest homology with O. pseudintermedium (99%), which indicated the strain was a member of O. pseudintermedium. This revealed for the first time that Ochrobactrum species could degrade polycyclic aromatic hydrocarbon, which would guide the biotreatment of such environmental contaminant with the bacterium.
Date of Conference: 16-18 May 2008