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Plastic substrate based display could potentially be widely used in electronic devices. Although with many advantages, it may potentially fail because of temperature induced stresses, or structural loads, or material damage resulted from the other environment factors in the practical use. On the other hand, as a relatively new display, the material behavior of the plastic substrate based display has been well understood. In many cases, there is no report of the material properties of those in the display assembly. In this study, we proposed an alternative method to estimate the material properties in the display assembly using experiments and FEM simulations. With the material data from this methodology, the thermal-structural analysis of the plastic substrate based display was performed using FEA. The results matches well with those from the experiments.