By Topic

Proteomic Analysis of a Response to Long-Chain Fatty Acid in Escherichia coli and Its Application

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$33 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

4 Author(s)
Mee-Jung Han ; Dept. of Chem. & Biomol. Eng., Korea Adv. Inst. of Sci. & Technol., Daejeon ; Jeong Wook Lee ; Sang Yup Lee ; Jong Shin Yoo

A long-chain fatty acid is a regulatory signal that modulates gene expression. In this study, proteomic studies that compared global translational differences between Escherichia coli cells grown in the presence of exogenous glucose and oleic acid were examined by two-dimensional gel electrophoresis (2-DE) and tandem mass spectrometry (LC-MS/MS). Of about 40 proteins showing altered expression levels with the presence of oleic acid, 10 including AldA, AceA, FadA, FadB, FadL, MalE, RbsB, Udp, Cdd and YccU were newly synthesized. Among them, the promoter of aldehyde dehydrogenase A (aldA promoter) was used for the production of a recombinant protein. Analysis of fluorescence intensities and confocal microscopic imaging revealed that aldA promoter is a very good promoter in that it is tightly controlled and highly expressed with active form compared with IPTG- inducible promoter. Thus the aldA promoter induced by oleic acid may be useful for achieving high-level expression of genes in E. coli.

Published in:

Frontiers in the Convergence of Bioscience and Information Technologies, 2007. FBIT 2007

Date of Conference:

11-13 Oct. 2007