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Main research in this paper is the DA(data acquisition) system design which based on the USB and MMC. According a USB's chip correlation hardware information, USB protocol, DA systematic structure and singlechip computer information, a hardware development solution was come out in the design. This solution consists of the data acquisition module hardware development design, singlechip computer data acquisition program development , USB's installation interface design and debug, embedded software(SW) and file system design. Finally we achieves the DA system which is more speedly, without delay and more dependable.