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This paper reports on an implementation of a novel valve based on the venous valve principle for complex microfluidic networks. Strong coupled field numerical simulations were used to analyse flexible artificial micro-machined venous valves for the first time. Different designs are optimized towards a maximum pressure distribution onto the flaps of the valves to achieve a higher diodicity. The simulation illustrated mechanisms to elevate the diodicities of the valves. The diodicities of the valves were characterised using fluorescence-based measurements, which enables noncontact determination of micro-valve diodicities and volume flows.