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Injection and cutting methods of animal cells using a microfluidic chip

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5 Author(s)
Ichikawa, A. ; Adv. Ind. Sci. & Technol., Indore ; Takahashi, S. ; Matsukawa, K. ; Tanikawa, T.
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We have developed a novel technique for cutting animal cells for an automatic cloning system. Animal cells that have been chemically softened are aspirated into a narrow Teflon tube, which is then inserted into a microfluidic chip containing two orthogonal channels. One of the microchannels aspirates and fixes a cell, while the other cuts the cell with a high velocity fluid flow. The thickness of the aspiration channel is adjustable. Half the volume of the cell is aspirated into the narrow microchannel and high-speed flow is then generated to cut the cell in two. In this report, we describe the cutting and injection methods.

Published in:

Micro Electro Mechanical Systems, 2008. MEMS 2008. IEEE 21st International Conference on

Date of Conference:

13-17 Jan. 2008