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Micro beads were patterned in microfluidic channels by using standing surface acoustic waves (SSAW). The SSAW were formed through two parallel interdigital transducers (IDTs) on a LiNbO3 substrate. A PDMS microchannel was aligned with the IDTs and bonded with the substrate. A solution of fluorescent polystyrene beads (diameter: 1.9 mum) was injected into the channel through a pressure driven flow. When a 25 dBm AC signal (frequency: 23.9 MHz) was applied to the IDTs, the beads were patterned into two straight lines (~5 mum in width) along the pressure nodes inside the channel.