Cart (Loading....) | Create Account
Close category search window
 

In-situ Single Cell Mechanics Characterization of W303 Yeast Cells using Nanoneedles inside Environmental-SEM

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

5 Author(s)

In this paper, the characterizations of cellular mechanics of W303 yeast cells have been conducted using nanoneedles inside environmental-SEM (ESEM). This enhanced ESEM system comprises of a standard ESEM instrument as a nano imaging tool, a cooling stage as a cellular biology's humidity controller and 7 D.O.F. linear actuators as nanomanipulator/effector. Four types of nanoneedles have been used in the experiments, i.e. silicon (Si) nanoneedle, titanium (Ti) coated Si nanoneedle and tungsten (W) nanoneedles. The former two nanoneedles are fabricated using 2 N/m spring constant's cantilevers. While the latter nanoneedles are fabricated using 0.09 N/m and 2 N/m spring constant's cantilevers (W0.09 and W2 nanoneedles). All types of the nanoneedles, i.e. Si, Ti-Si, W0.09 and W2 nanoneedles are able to be used for single cells' local stiffness characterizations. This ability can be used in future fast disease detection where the disease cells may shows different cell mechanics properties compare to the normal cell. In addition to this, the Si-Ti and W2 nanoneedles have shown an ability to penetrate the cell without cell bursting. This ability is important in the future single cell surgery where the cell damage can be eliminated as much as possible.

Published in:

Micro-NanoMechatronics and Human Science, 2007. MHS '07. International Symposium on

Date of Conference:

11-14 Nov. 2007

Need Help?


IEEE Advancing Technology for Humanity About IEEE Xplore | Contact | Help | Terms of Use | Nondiscrimination Policy | Site Map | Privacy & Opting Out of Cookies

A not-for-profit organization, IEEE is the world's largest professional association for the advancement of technology.
© Copyright 2014 IEEE - All rights reserved. Use of this web site signifies your agreement to the terms and conditions.