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Speech endpoint detection is an important step in the field of speech analysis, speech synthesis and speech recognition. This paper proposed an endpoint detection algorithm, which used amplitude entropy, spectral entropy and frame energy as feature parameters and utilized RBF neural network as a feature classification system. 170 sentences are used as testing data to detect speech endpoint, which length is from 4 second to 7 second. The experiments show that the testing results using RBF neural network are better than that using entropy alone or BP neural network based on entropy.