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We consider an orthogonal space-frequency block coded (OSFBC) orthogonal frequency-division multiplexing (OFDM) system under multi-tone interference and multi-path fading environment. An approximate analytic expression for the bit error probability of OSFBC-OFDM system with a block code matrix for four transmit antennas is derived and the derivation methodology for other code matrices is summarized. The bit error rate simulations are performed for QPSK signals and compared with the theoretical results. The influences of the total energy of multi-tone interference and the fraction of interfered bandwidth are investigated.