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In order to detect DNA, we employed nano magnetite powders of Fe3O4. In XRD results, the nanoparticle was synthesized mainly in magnetite phase and also maghemite (r-Fe2O3) phase was trivial portion. The surface morphology of the powders observed that the particle size was about 40 nm and did not change as varying annealing temperature. In agarose gel electrophoresis analysis, after bonding DNA, Fe2O3 and Fe3O4 nanopaticles showed the fixed state in the range of lane 2-4. But the mixed particles were very weak reaction when compared with Fe3O4. Additionally, Fe3O4 presented the reaction of DNA in lane 5~7. From these results, we confirmed a chance of DNA detection by using the nanoparticle.