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This study introduced a localized approach for magnetic resonance microscopy (MRM) of adult zebrafish brain on our 3T MRI system. A micro-fabricated single-channel radiofrequency (RF) receiver coil was designed by using electromagnetic simulation. Using transmit-only and receive-only (TORO) configuration, receive-only surface coil can achieve high signal-to-noise ratio (SNR) at localized brain regions. To demonstrate the capability, high-resolution MRI of the zebrafish brain with a spatial resolution of 78 times 78 times 500 mum was acquired. The advantages of using micro-fabricated coils on microscopy and molecular imaging were discussed.