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Chemical solution deposition (CSD) is used to prepare LaMnO (LMO) buffer layers on different substrates. The results show that biaxially oriented LMO films can be successfully prepared on single-crystal SrTiO3 (STO) and STO buffered single-crystal LaAlO3 substrates when humid 4%H2-N2 annealing atmosphere is used. The orientation of LMO-Ni is (110)-oriented even when the annealing atmosphere is humid 4%H2-N2. When CSD-derived STO-Ni is used as a template, biaxially oriented LMO buffer layers with c-axis orientation can be successfully prepared. The results provide an effective route to prepare LMO-based buffer layers using CSD.