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We investigated biquadratic exchange coupling strength in sputtered uniaxial antiferromagnetic Co/Ru/Co trilayers, which can be tuned by applying a magnetic field perpendicular to the sample surface during deposition. The perpendicular field induces a columnar grain structure that results in a perpendicular magnetic anisotropy for single Co layers. The perpendicular magnetic anisotropy is detected by combined magnetization and Brillouin light scattering measurements. In trilayers, increasing the perpendicular field during deposition results in a monotonic increase of the biquadratic exchange coupling strength, so these samples provide an experimental realization of the effect of variable biquadratic coupling strength on magnetic hysteresis behavior. The hard-axis remanence is a direct measure of biquadratic exchange coupling strength, which we demonstrated both by experiment and by a Stoner-Wohlfarth model that includes the relevant magnetic parameters.