By Topic

Segmentation and Detection of Nuclei in Silver Stained Cell Specimens for Early Cancer Diagnosis

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$33 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

5 Author(s)
Andre A. Bell ; Institute of Imaging and Computer Vision, RWTH Aachen University, 52056 Aachen, Germany. Email: andre.bell@lfb.rwth-aachen.de ; Gerlind Herberich ; Dietrich Meyer-Ebrecht ; Alfred Bocking
more authors

For successful cure, cancer has to be detected as early as possible. Since cancer starts from a single cell, this can best be done using cytopathological methods. One important diagnostically relevant measure is the proliferation rate of the cells, which can be estimated from segmented silver stained nuclei. However, the microscopy images of silver stained specimens vary strongly in intensity and contrast and are furthermore compromised by an overall texture. We show that a precise segmentation of the nuclei is possible using a two-step approach. First, an oversegmentation with the mean shift algorithm is obtained. In a second step, these regions are merged to objects, guided by a suitable shape model, viz an ellipse, but simultaneously allowing deviations from this shape model. The segmentation results are compared to a gold standard of 8617 nuclei from 23 specimens of the thyroid gland, achieving a mean areal segmentation error of DeltaA macrnucleus = 12mum2 per nucleus.

Published in:

2007 IEEE International Conference on Image Processing  (Volume:6 )

Date of Conference:

Sept. 16 2007-Oct. 19 2007